5nm NHS-Activated Gold Nanoparticle Conjugation Kit (3 Reactions)

Cytodiagnostics 5nm NHS-activated Gold Nanoparticle Conjugation Kit have been optimized for high efficiency one-step covalent conjugation of proteins and other primary amine-containing ligands to gold nanoparticles.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester gold nanoparticles supplied in the kit. 

This mini kit contains 3 individual reaction vials with lyophilized NHS-activated gold nanoparticles for performing three independent conjugation reactions.

Kit Components

• 5nm NHS-Activated Gold Nanoparticles (lyophilized) - 3 vials
• Protein Resuspension Buffer - 1.5ml
• Reaction Buffer - 1.5ml
• Quencher Solution - 1.5ml

Features & Benefits

• Results in covalently bound ligand and more stable conjugate.
• Fast and convenient one-step conjugation reaction with no pre-activation requirements.
• Spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
• Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

Gold Nanoparticle Specifications

Surface functionality: NHS-ester (~ 1 NHS group/nm2) (5kDa PEG-spacer between gold surface and NHS-group)

Core diameter: 5nm 

Concentration: OD=20 (when dissolved to a final volume of 100ul)

Particles per ml: ~1.1E+15 (when dissolved to a final volume of 100ul)

Absorbance (lambda max): 515-520nm