MWCO Ultrafiltration Spin Columns, 30 kDa, 0.5ml
MWCO Ultrafiltration Spin Columns, 30 kDa, 0.5ml
MWCO Ultrafiltration Spin Columns, 30 kDa, 0.5ml

MWCO Ultrafiltration Spin Columns, 30 kDa, 0.5ml

Product Description

Sample cleanup, desalting, concentrating centrifugal filters. Simple one spin for rapid concentrating sample, and removal of small contaminants (salt, dyes, radioactive labels). Polyethersulfone (PES) membrane for maximum recovery of protein and DNA.

Ultrafiltration spin units offer a simple, one step procedure for desalting and concentration of proteins and other macromolecules with greater than 90% recovery. Utilizing molecular weight cut-off polyethersulfone (PES) filter membrane, the spin device is able to retain macromolecules having the greater MW, and filtrate salts and other molecules of the smaller MW. Because of low protein/DNA binding properties of the PES filter, loss of the retentate on the filter is minimized. The retentate can be recovered at excellent rate.

Four molecular weight cut-off (MWCO) sizes are provided to cover a broad range of molecular weights: 10 K, 30 K, 50 K, and 100 K. It is recommended to choose an MWCO 1/2 to 1/3 smaller than the protein to be concentrated. For instance, to concentrate a protein of 80 kDa, we recommend to start with the 30 K MWCO spin unit.

Product Features

  • Simple, rapid spin for simultaneous desalting and concentrating (up to 75-fold concentration);
  • Buffer exchange in two quick steps;
  • Faster filtration - high flux PES membrane is heat-sealed to the housing unit;
  • High recovery of the retentate - the PES membrane has very low absorption property for protein and DNA/RNA and the filter housing unit is also constructed with low-binding materials;
  • Feasibility of collecting the filtrate - it is flexible to choose either the retentate or the filtrate to keep;
  • Optimized designs for better performance:
  1. Built-in dead stop pocket at the filter unit bottom - no risk of dryout during spin;
  2. Vertical position of the filter membrane - less clogging/fouling issue;
  • Less processing time, less buffers, and less mess compared to dialysis;
  • Flexible throughput - it is easy to perform multiple samples in parallel or to scale up with the same sample;
  • Free of detectable DNase/RNase contamination.

Applications:

  • Concentration, desalting of proteins, enzymes, antibodies, DNA/RNA and other macromolecules

  • Rapid removal of small contaminants (salts, dyes, detergent, and additives)

  • Post-modification removal of labeled amino acids and nucleotides, or other unincorporated labels

  • Sample pre-treatment prior to HPLC

  • Deproteinization

  • Recovery of biomolecules from cell culture supernatants and cell lysates

  • Concentrating virus from cell culture supernatants